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Biotinylation Reagents

Biotinylation Reagents

Biotin-Streptavidin is among the strongest interactions found in nature. Biotinylation reagents are widely used to enable the isolation, separation, concentration and further downstream processing or analysis of biomolecules. Appropriate biotinylation of the target molecule is essential to ensure high binding efficiency to streptavidin or Immobilized Streptavidin. ProteoChem manufactures an assortment of biotinylation reagents that target various functional groups like primary amines, sulfhydryls (thiols), carboxyls, and carbohydrates.

ProteoChemís biotinylation reagents are offered as water soluble (Sulfo-NHS ester containing) or insoluble varieties. ProteoChem also offers biotins with diverse spacer arm lengths which can be used to reduce steric hindrances associated with streptavidin binding, thus allowing for more efficient binding of biotinylated proteins and peptides.

Biotinylation Reagent Selection Guide
Crosslinker
Acronym
Spacer Arm
Length
M.W. Reactivity Cleavable
By
Membrane
Permeable
Water
Soluble

NHS-Biotin

13.5 Å

341.4

-NH2 - Yes No
NHS-LC-Biotin 22.4 Å 454.5 -NH2 - Yes No

NHS-SS-Biotin

24.3 Å 504.7 -NH2 Thiols Yes No

Sulfo-NHS-Biotin

13.5 Å 443.4 -NH2 - No Yes
Sulfo-NHS-LC-Biotin 22.4 Å 556.6 -NH2 - No Yes

Sulfo-NHS-SS-Biotin

24.3 Å 606.7 -NH2 Thiols No Yes
Biotin-HPDP 29.2 Å 539.8 -SH2 Thiols Yes No
Iodoacetyl-LC-Biotin 27.1 Å 510.4 -SH2 - Yes No
Biotin Hydrazide 15.7 Å 258.3 -CHO - Yes No
D-Biotin 13.5 Å 244.3 -NH2* - No Yes

Please follow the links below to see pricing and product information:

Sulfo-NHS-Biotin
Sulfo-NHS-Biotin
NHS-Biotin
NHS-Biotin
Sulfo-NHS-LC-Biotin
Sulfo-NHS-LC-Biotin
NHS-LC-Biotin
NHS-LC-Biotin
Sulfo-NHS-SS-Biotin
Sulfo-NHS-SS-Biotin
NHS-SS-Biotin
NHS-SS-Biotin
Biotin Hydrazide
Biotin Hydrazide
Biotin-HPDP
Biotin-HPDP
Iodoacetyl-LC-Biotin
Iodoacetyl-LC-Biotin
D-Biotin
D-Biotin
BMCC-Biotin
BMCC-Biotin
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