BS2G crosslinker (BS2G; Bis[Sulfosuccinimidyl] glutarate) is an amine-reactive, water soluble, homobifunctional protein crosslinker. The BS2G protein crosslinker's 5-atom (7.7 angstrom) spacer arm is non-cleavable and the molecule is not cell membrane permeable. The BS2G crosslinking reagent's Sulfo-NHS esters are amino reactive and can be used to label cell surface proteins. Also, the Sulfo-NHS esters of BS2G allow the crosslinking reaction to occur in aqueous solutions in near physiological conditions. DSG (Disuccinimidyl glutarate; Di(N-succinimidyl) glutarate) is the membrane permeable analog of BS2G.
BS2G is the light version (BS2G-d0), whereas BS2G-d4 is the heavy, deuterated analog and together they can be used for protein-protein interaction mapping using Liquid Chromatography–Mass Spectrometry (LCMS, LC/MS, LC-MS).
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Molecular Weight: 530.35
Spacer Length: 7.7 Å
Alternative Names: Glutaric acid bis(3-sulfo-N-hydroxysuccinimide ester); BS2G; Sulfo-DSG; BS2G cross-linker; BS2G-d0; BS2G-d0
Kannan, R., Santhoshkumar, P., Mooney, B. P., & Sharma, K. K. (2013). αA66-80 peptide interacts with soluble α-crystallin and induces its aggregation and precipitation: A contribution to age related cataract formation. Biochemistry.
Kannan, R., Santhoshkumar, P., Mooney, B. P., & Sharma, K. K. (2013). Identification of Subunit-Subunit Interaction Sites in αA-WT Crystallin and Mutant αA-G98R Crystallin Using Isotope-Labeled Cross-Linker and Mass Spectrometry. PloS one, 8(6), e65610.
Special bulk pricing is available on bulk quantities of BS2G crosslinker for your large volume manufacturing application.