Disuccinimidyl suberate (DSS) is a homobifunctional crosslinker that is cell membrane permeable. DSS crosslinking reagent has amine-reactive NHS esters at both ends of an 8-atom (11.4 angstrom) spacer arm. DSS protein crosslinker must be dissolved in an organic solvent, such as DMSO or DMF, then added to an aqueous crosslinking reaction. The DSS solution often becomes slightly hazy in an aqueous mixture.
DSS crosslinking reagent can be used to crosslink intracellular proteins. If extracellular crosslinking is desired, then BS3, the water soluble analog of DSS can be used.
Purity (NMR): ≥ 95% (typical lots are 99% pure)
Visual: White to off-white powder
Melting Point: 162-170ºC
Click here for: DSS Crosslinking Protocol & Product Information Sheet
Molecular weight. 368.34
Spacer Length: 11.4 Å
CAS Number: 68528-80-3
Alternative name: Suberic acid bis(N-hydroxysuccinimide ester)
Special bulk pricing is available on bulk quantities of DSS for your large volume manufacturing application.