LC-SPDP crosslinker, Succinimidyl 6-(3-[2-pyridyldithio]-propionamido)hexanoate, is a cleavable, water insoluble, amino and thiol (sulfhydryl) reactive heterobifunctional protein crosslinker. The "long chain" LC-SPDP crosslinking reagent has a 15.7 angstrom spacer arm, compared to the shorter 6.8 angstrom spacer arm version of SPDP crosslinker. Often, due to steric hindrances, it is desirable to empirically test which of the two LC-SPDP crosslinker versions will work best in your specific crosslinking application.
LC-SPDP crosslinker's reactive moites consist of an N-hydroxysuccinimide (NHS) ester that reacts with primary amines, as found in lysine side chains. While on the other end of the LC-SPDP crosslinker molecule pyridinyldisulfide reacts with sulhydryls to yield a reducible disulfide bond which can be reduced with TCEP-HCl or DTT to reverse the crosslinked proteins.
Molecular Weight: 425.5
Spacer Length: 15.7 Å
Special bulk pricing is available on bulk quantities of LC-SPDP crosslinking reagent for your large volume manufacturing application.