p-Nitrophenylphosphate (pNPP) is a chromogenic substrate for alkaline phosphatase (AP) in ELISA and other immunoassays. Under AP influence pNPP decays to yellow para-nitrophenol which is then measured spectrophotometrically at 405 nm. pNPP is supplied as a powder and is stored frozen.
Molecular Weight: 371.14
CAS Number: 4264-83-9
Alternative Names: para-Nitrophenylphosphate; p-Nitrophenyl phosphate disodium hexahydrate; para-Nitro Phenyl Phosphate; PNPP