Protein
crosslinkers are
chemical compounds used to
covalently bind, or conjugate,
biomolecules together. In
proteomics, "protein crosslinking"
refers to utilizing protein
crosslinkers to conjugate
peptides or proteins together
or to immobilize proteins to a
solid support.
Protein crosslinking
agents possess reactive
moieties specific to various
functional groups
(sulfhydryls, amines,
carbohydrates, etc.) on
proteins, peptides, or other
molecular complexes. The
atoms separating a
crosslinker reagent’s reactive
groups,
and eventually the conjugated
proteins, form the “spacer
arm”. A zero-length
crosslinker refers to protein
crosslinkers that join two
molecules without adding
additional spacer arm atoms.
Homobifunctional
crosslinker reagents have
the same reactive group on
both ends of the
spacer arm
(i.e., Amine Reactive-Amine
Reactive); while
heterobifunctional
crosslinkers have different
reactive groups on each end of
a spacer arm (i.e., Sulfhydryl
Reactive-Amine Reactive).
Why use protein
crosslinkers?
Protein crosslinking has
become an important tool for the
preparation of bioconjugates
used in immunoassays, isotope
labeled crosslinkers are
used for probing protein-protein
interactions by mass
spectrometry, as well as other
creative crosslinking
methodologies that include:
· Determining protein
structure or spatial
orientation of a protein
· Studying protein-protein
interactions
· Forming carrier
protein-hapten conjugates
· Producing antibody-protein
crosslinked conjugates
· Covalently crosslinking proteins
to a solid surface
· Creating antibody-enzyme or
fluorophore-peptides
conjugates
· Analyzing enzyme activity
and protein degradation
Don't let reagent costs pierce
your crosslinker budget.
ProteoChem manufactures
ultrapure, world-class
crosslinkers at an exceptional
value. We guarantee our
protein crosslinking reagents
to work in your proteomics
research and stand behind our
products.
Please click on the following
links to browse the complete
list of protein crosslinking
products offered in each category:
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