Zero-Length Crosslinkers - EDC-HCl, Sulfo-NHS & NHS       
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Zero-Length Crosslinkers - EDC-HCl, Sulfo-NHS & NHS

Zero-Length Crosslinkers

Zero-length crosslinkers form protein conjugates without adding additional spacer arm atoms between two conjugated molecules. The crosslinker 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC-HCl or EDAC-HCl) is commonly used for peptide synthesis, preparation of immunoconjugates, and protein crosslinking. EDC-HCl carbodiimide is routinely used in concert with N-hydroxysuccinimide (NHS) or N-hydroxysulfosuccinimide (Sulfo-NHS) to enhance EDC-HCl coupling efficiency by acting as a catalyst.

Zero-length Crosslinker Selection Guide
Crosslinker
Acronym
Spacer Arm
Length
M.W. Reactivity Cleavable
By
Membrane
Permeable
Water
Soluble
CDI 0.0 Å 162.2 -NH2 ; -COOH - n/a *reacts
DCC 0.0 Å 206.3 -NH2 ; -COOH - Yes No
EDC-HCl 0.0 Å 191.7 -NH2 ; -COOH - No Yes
NHS 0.0 Å 115.1 -NH2 ; -COOH - Yes No
Sulfo-NHS 0.0 Å 217.1 -NH2 ; -COOH - No Yes

Please follow the links below to see pricing and product information:

EDC-HCl (EDAC-HCl)
EDC-HCl (EDAC-HCl)
NHS
NHS
Sulfo-NHS
Sulfo-NHS
CDI
CDI
DCC
DCC
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