Zero-Length Crosslinkers - EDC-HCl, Sulfo-NHS & NHS

Zero-length crosslinkers form protein conjugates without adding additional spacer arm atoms between two conjugated molecules. The crosslinker 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC-HCl or EDAC-HCl) is commonly used for peptide synthesis, preparation of immunoconjugates, and protein crosslinking. EDC-HCl carbodiimide is routinely used in concert with N-hydroxysuccinimide (NHS) or N-hydroxysulfosuccinimide (Sulfo-NHS) to enhance EDC-HCl coupling efficiency by acting as a catalyst.

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