Sulfo-NHS-LC-Biotin, Sulfosuccinimidyl 6-(biotinamido) Hexanoate, is a long chain, water soluble biotinylation reagent that is reactive toward primary amine groups. Similar to Sulfo-NHS-Biotin, Sulfo-NHS-LC-Biotin is designed to target cell surface proteins. The sodium sulfonate groups lend water solubility to Sulfo-NHS-LC-Biotin and prevent it from crossing the lipid bi-layer of the cell membrane; whereas NHS-LC-Biotin can biotinylate intracellular proteins. The resulting biotinylated protein can then be detected when used in conjunction with streptavidin and Immobilized Streptavidin.
The "long chain" Sulfo-NHS-LC-Biotin and Sulfo-NHS-Biotin both have the same chemical functionality. However, Sulfo-NHS-LC-Biotin will provide a longer spacer arm that is designed to overcome any steric hindrances that may be present between the labeled protein(s) and streptavidin (ProteoChem product # e7105-5mg). Often, the best results are determined by empirically testing each reagent for your specific application.
Click here for: Sulfo-NHS-LC-Biotin Product Information Sheet & General Protocol
Molecular Weight: 556.6
Spacer Length: 22.4 Å
CAS Number: 127062-22-0
Alternative Names: Biotin-X-NHS, Water-Soluble; Biotinamidohexanoic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt; Sulfo NHS LC Biotin; sulfosuccinimidyl biotinamido hexanoate; sulfo succinimidyl biotinamido hexanoate; Biotinamidocaproate N-hydroxysulfosuccinimidyl ester; sulfosuccinimidyl biotinamidyl hexanoate; long chain Sulfo NHS Biotin
Senchenkova, E. Y., Komoto, S., Russell, J., Almeida-Paula, L. D., Yan, L. S., Zhang, S., & Granger, D. N. (2013). Interleukin-6 Mediates the Platelet Abnormalities and Thrombogenesis Associated with Experimental Colitis. The American journal of pathology.
Special bulk pricing is available on bulk quantities of Sulfo-NHS-LC-Biotin for your large volume manufacturing application.