Sulfo-SANPAH crosslinker (N-Sulfosuccinimidyl-6-[4'-azido-2'-nitrophenylamino] hexanoate) is a water soluble, heterobifunctional protein crosslinker with a spacer arm length of 18.2 angstroms. The Sulfo-SANPAH crosslinking reagent contains an NHS ester that reacts with primary amines and a nitrophenylazide group that is photoreactive at 320-350 nm toward amino groups. Sulfo-SANPAH crosslinker is immediately water soluble without the use of organic solvents. Commonly, Sulfo-SANPAH enhances Western blot experiments; likewise Sulfo-SANPAH activated polyacrylamide (PAA) gel crosslinked to fibronectin or collagen extracellular matrix (ECM) is used in tissue culture studies.
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Molecular Weight: 492.40
Spacer Length: 18.2 Ĺ
CAS Number: 102568-43-4
Alternative Names: 1-[6-[(4-azido-2-nitro-phenyl)amino]hexanoyloxy]-2,5-dioxo-pyrrolidine-3-sulfonic acid; Sulfosuccinimidyl 6-((4-azido-2-nitrophenyl)amino)hexanoate
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