Heterobifunctional Crosslinkers

Heterobifunctional crosslinkers are probably the most notable and useful protein crosslinking reagents because they have two distinct reactive groups. This allows the protein conjugation reaction to progress in a controlled, two-step reaction; thereby avoiding the formation of dimers and polymers while crosslinking proteins.

Heterobifunctional Crosslinker Selection Guide

Crosslinker Acronym	Spacer Arm Length	M.W.	Reactivity	Cleavable By	Membrane Permeable	Water Soluble
ANB-NOS	7.7 Å	305.2	-NH2 ; -NH2, -C=C-, -CH, -NH	-	Yes	No
BMPS	5.9 Å	266.2	-NH2 ; -SH2	-	Yes	No
EMCS	9.4 Å	308.3	-NH2 ; -SH2	-	Yes	No
GMBS	7.3 Å	280.2	-NH2 ; -SH2	-	Yes	No
LC-SPDP	15.7 Å	425.5	-NH2 ; -SH2	Thiols	Yes	No
MBS	7.3 Å	314.3	-NH2 ; -SH2	-	Yes	No
PDPH	9.2 Å	229.3	-SH2;-CHO	Thiols	Yes	Yes
SBA	1.5 Å	236.0	-NH2 ; -SH2	-	Yes	No
SIA	1.5 Å	283.0	-NH2 ; -SH2	-	Yes	No
Sulfo-SIA	1.5 Å	385.1	-NH2 ; -SH2	-	No	Yes
SMCC	8.3 Å	334.3	-NH2 ; -SH2	-	Yes	No
SMPB	11.6 Å	356.3	-NH2 ; -SH2	-	Yes	No
SMPH	14.3 Å	379.4	-NH2 ; -SH2	-	Yes	No
SPDP	6.8 Å	312.4	-NH2 ; -SH2	Thiols	Yes	No
Sulfo-LC-SPDP	15.7 Å	527.6	-NH2 ; -SH2	Thiols	No	Yes
Sulfo-MBS	7.3 Å	416.3	-NH2 ; -SH2	-	No	Yes
Sulfo-SANPAH	18.2 Å	492.4	-NH2 ; -NH2, -C=C-, -CH, -NH	-	No	Yes
Sulfo-SMCC	8.3 Å	436.4	-NH2 ; -SH2	-	No	Yes

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