Heterobifunctional Crosslinkers       
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Heterobifunctional Crosslinkers

Heterobifunctional Crosslinkers

Heterobifunctional crosslinkers are probably the most notable and useful protein crosslinking reagents because they have two distinct reactive groups. This allows the protein conjugation reaction to progress in a controlled, two-step reaction; thereby avoiding the formation of dimers and polymers while crosslinking proteins.

Heterobifunctional Crosslinker Selection Guide
Crosslinker
Acronym
Spacer Arm
Length
M.W. Reactivity Cleavable
By
Membrane
Permeable
Water
Soluble
ANB-NOS 7.7 Å 305.2 -NH2 ;
-NH2, -C=C-, -CH, -NH
- Yes No
BMPS 5.9 Å 266.2 -NH2 ; -SH2 - Yes No
EMCS 9.4 Å 308.3 -NH2 ; -SH2 - Yes No
GMBS 7.3 Å 280.2 -NH2 ; -SH2 - Yes No
LC-SPDP 15.7 Å 425.5 -NH2 ; -SH2 Thiols Yes No
MBS 7.3 Å 314.3 -NH2 ; -SH2 - Yes No
PDPH 9.2 Å 229.3 -SH2;-CHO Thiols Yes Yes
SBA 1.5 Å 236.0 -NH2 ; -SH2 - Yes No
SIA 1.5 Å 283.0 -NH2 ; -SH2 - Yes No
Sulfo-SIA 1.5 Å 385.1 -NH2 ; -SH2 - No Yes
SMCC 8.3 Å 334.3 -NH2 ; -SH2 - Yes No
SMPB 11.6 Å 356.3 -NH2 ; -SH2 - Yes No
SMPH 14.3 Å 379.4 -NH2 ; -SH2 - Yes No
SPDP 6.8 Å 312.4 -NH2 ; -SH2 Thiols Yes No
Sulfo-LC-SPDP 15.7 Å 527.6 -NH2 ; -SH2 Thiols No Yes
Sulfo-MBS 7.3 Å 416.3 -NH2 ; -SH2 - No Yes
Sulfo-SANPAH 18.2 Å 492.4 -NH2 ;
-NH2, -C=C-, -CH, -NH
- No Yes
Sulfo-SMCC 8.3 Å 436.4 -NH2 ; -SH2 - No Yes

Please follow the links below to see pricing and product information:

SMCC
SMCC
Sulfo-SMCC
Sulfo-SMCC
Sulfo-SANPAH
Sulfo-SANPAH
SMPH
SMPH
PDPH
PDPH
MBS
MBS
Sulfo-MBS
Sulfo-MBS
SPDP
SPDP
LC-SPDP
LC-SPDP
Sulfo-LC-SPDP
Sulfo-LC-SPDP
SIA
SIA
Sulfo-SIA
Sulfo-SIA
SBA
SBA
SMPB
SMPB
BMPS
BMPS
EMCS
EMCS
GMBS
GMBS
ANB-NOS
ANB-NOS
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