Zero-Length Crosslinkers - EDC-HCl, Sulfo-NHS & NHS

Zero-length crosslinkers form protein conjugates without adding additional spacer arm atoms between two conjugated molecules. The crosslinker 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC-HCl or EDAC-HCl) is commonly used for peptide synthesis, preparation of immunoconjugates, and protein crosslinking. EDC-HCl carbodiimide is routinely used in concert with N-hydroxysuccinimide (NHS) or N-hydroxysulfosuccinimide (Sulfo-NHS) to enhance EDC-HCl coupling efficiency by acting as a catalyst.

Zero-length Crosslinker Selection Guide

Crosslinker Acronym	Spacer Arm Length	M.W.	Reactivity	Cleavable By	Membrane Permeable	Water Soluble
CDI	0.0 Å	162.2	-NH2 ; -COOH	-	n/a	*reacts
DCC	0.0 Å	206.3	-NH2 ; -COOH	-	Yes	No
EDC-HCl	0.0 Å	191.7	-NH2 ; -COOH	-	No	Yes
NHS	0.0 Å	115.1	-NH2 ; -COOH	-	Yes	No
Sulfo-NHS	0.0 Å	217.1	-NH2 ; -COOH	-	No	Yes

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