Biotinylation Reagents

Biotin-Streptavidin is among the strongest interactions found in nature. Biotinylation reagents are widely used to enable the isolation, separation, concentration and further downstream processing or analysis of biomolecules. Appropriate biotinylation of the target molecule is essential to ensure high binding efficiency to streptavidin or Immobilized Streptavidin. ProteoChem manufactures an assortment of biotinylation reagents that target various functional groups like primary amines, sulfhydryls (thiols), carboxyls, and carbohydrates.

ProteoChem’s biotinylation reagents are offered as water soluble (Sulfo-NHS ester containing) or insoluble varieties. ProteoChem also offers biotins with diverse spacer arm lengths which can be used to reduce steric hindrances associated with streptavidin binding, thus allowing for more efficient binding of biotinylated proteins and peptides.

Biotinylation Reagent Selection Guide

Crosslinker Acronym	Spacer Arm Length	M.W.	Reactivity	Cleavable By	Membrane Permeable	Water Soluble
NHS-Biotin	13.5 Å	341.4	-NH2	-	Yes	No
NHS-LC-Biotin	22.4 Å	454.5	-NH2	-	Yes	No
NHS-SS-Biotin	24.3 Å	504.7	-NH2	Thiols	Yes	No
Sulfo-NHS-Biotin	13.5 Å	443.4	-NH2	-	No	Yes
Sulfo-NHS-LC-Biotin	22.4 Å	556.6	-NH2	-	No	Yes
Sulfo-NHS-SS-Biotin	24.3 Å	606.7	-NH2	Thiols	No	Yes
Biotin-HPDP	29.2 Å	539.8	-SH2	Thiols	Yes	No
Iodoacetyl-LC-Biotin	27.1 Å	510.4	-SH2	-	Yes	No
Biotin-BMCC	15.7 Å	533.7	-SH2	-	Yes	No
Biotin Hydrazide	15.7 Å	258.3	-CHO	-	Yes	No
D-Biotin	13.5 Å	244.3	-NH2*	-	No	Yes

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