BS2G-d4 deuterated crosslinker, Bis(Sulfosuccinimidyl) 2,2,4,4-glutarate-d4, is a water soluble and cell membrane impermeable, protein crosslinker containing 4 deuterium atoms to provide a 4 dalton shift by mass spectrometry analysis. Use heavy BS2G-d4 deuterated crosslinking reagent in combination with the non-deuterated analog BS2G-d0 (BS²G-d₀, BS2G) for mass spec studies. Together BS2G-d0 and BS2G-d4 can be used for protein-protein interaction mapping using Liquid Chromatography–Mass Spectrometry (LCMS, LC/MS, LC-MS).

Molecular Weight: 534.37

Spacer Length: 7.7Å

Alternative Names: Glutaric acid-2,2,4,4-d4 bis (3-sulfo-N-hydroxysuccinimide ester) disodium salt; D4-BS2G; BS²G-d₄; d 4 -BS2G

References:
Kannan, R., Santhoshkumar, P., Mooney, B. P., & Sharma, K. K. (2013). The αA66–80 Peptide Interacts with Soluble α-Crystallin and Induces Its Aggregation and Precipitation: A Contribution to Age-Related Cataract Formation. Biochemistry, 52(21), 3638-3650.

Kannan, R., Santhoshkumar, P., Mooney, B. P., & Sharma, K. K. (2013). Identification of Subunit-Subunit Interaction Sites in αA-WT Crystallin and Mutant αA-G98R Crystallin Using Isotope-Labeled Cross-Linker and Mass Spectrometry. PloS one, 8(6), e65610.

Special bulk pricing is available on bulk quantities of BS2G-d4 for your large volume manufacturing or commercial application.