NHS-SS-Biotin, Succinimidyl-2-(biotinamido)-ethyl-1,3'-dithiopropionate, is an amine-reactive biotinylation reagent that is cleavable when the disulfide (-S-S-) bond is reduced. NHS-SS-Biotin is cell membrane permeable and has a long chain separating the biotin group from the amino-reactive NHS-ester (N-Hydroxysuccinimide ester). The resulting biotinylated protein can then be detected when used in conjunction with streptavidin.

It is possible to cleave the biotin group from the crosslinked target protein under reducing conditions, such as in the presence of beta-mercaptoethanol (BME), TCEP-HCl or DTT. This technique is often used to purify an antibody or other protein from a biotin specific affinity resin like Immobilized Streptavidin without exposing your protein of interest to denaturing conditions.

Click here for: NHS-SS-Biotin Protocol & Product Information Sheet