Sulfo-NHS-SS-Biotin biotinylation reagent, Sulfosuccinimidyl-2-(biotinamido)-ethyl-1,3'-dithiopropionate, is cleavable, water-soluble and amino-reactive. The Sulfo-NHS-SS-Biotin molecule is cell membrane impermeable due to its sodium sulfonate group. Sulfo-NHS-SS-Biotin has a long chain spacer arm separating the biotin group from the amino-reactive sulfo-NHS ester moity. The resulting biotinylated protein can then be detected when used in conjunction with streptavidin and Immobilized Streptavidin. It is possible to cleave the biotin group from the crosslinked target protein under reducing conditions, such as in the presence of 2-mercaptoethanol, TCEP-HCl or DTT.
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Molecular Weight: 606.69
Spacer Length: 24.3 Å
CAS Number: 325143-98-4
Alternative Names: Biotin disulfide N-Hydroxysulfosuccinimide ester; Sulfo NHS SS Biotin
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